Clickmer Systems
What are Clickmers®?
Synthetic affinity reagents known as Clickmers are chemically modified ssDNA oligonucleotides that, based on their sequence and modifications, form three-dimensional structures that adaptively bind to their target molecules with high affinity and specificity.
The introduction of side chains/modifications through the Nobel Prize-winning ‘Click’ chemistry provides increased chemical variability and flexibility compared to standard aptamers, which increases the probability of developing an excellent target binder.
What are the benefits of employing Clickmers in your technological platforms?
Chemical synthetic synthesis means improved batch-to-batch continuity
Improved turn-around time – Target-binding Clickmers can be developed within a few weeks with subsequent characterisation bespoke to the client’s requirements
Ethical development – no animal testing required
Flexible assay read-out: enzymatic, fluorescent or even RT-qPCR-based according to the client’s needs
Increased binding surface of the target protein landscape in comparison to custom/commercial antibodies raised against short peptides
A Nobel Prize Winning Technology - ‘Click’ Chemistry
APIS Assay Technologies have the knowledge and capability to develop Clickmer products using this Nobel Prize winning technology.
How are Clickmers selected?
Our proprietary Click-SELEX is an in vitro evolutionary process of iterative cycles for the selection of Clickmers, which are consequently isolated and enriched through PCR to generate a pool of specific target-binding Clickmers (see below).
A major benefit of the SELEX process is the ability to adapt the selection process to meet client requirements, e.g. by selecting a Clickmer in the presence of different mediums, including serum.
Click-SELEX is an in vitro evolutionary process of iterative cycles
Custom Clickmer Development
Clickmer development is available to integrate into our client’s platform, or to integrate as part of our Contract Assay Development service
Clickmers provide next-generation antibody-analogue tools that enable researchers and diagnostic developers to overcome the limitations of antibody-based technology and batch-to-batch variability
Our Clickmer Systems Development Service offers a structured milestone-defined development pipeline focused on understanding client requirements and project aims
Featured Clickmer Application - Protein Purification
Ideal reagents for sample preparation and affinity protein purification
Clickmers high binding specificity in complex matrices reduces the number of purification and refinement steps, and improves yield of highly pure protein for use in downstream applications.
As Clickmers do not bind to misfolded and denatured proteins, only native-like functional protein is purified. Furthermore, high quality of the purified protein is maintained by our proprietary, customised Clickmer selection process for conditional binding so that the protein can be eluted under non-denaturing conditions, which also simplifies column regeneration.
Thanks to the ease of covalent conjugation to resin such as Sepharose and the batch-to-batch continuity, Clickmers provide the means to purify your protein of choice reproducibly, at high quality.
Clickmer® Systems GmbH have been awarded the BSFZ Seal of Innovation Competence
Clickmer® Systems GmbH are honoured to receive the BSFZ Seal of Innovation Competence from the Federal Ministry of Education and Research for 2024. This recognition underscores our unwavering dedication to advancing innovation in the field of aptamers.
Our exceptional R&D team continues to pioneer new solutions in the discovery and development of our chemically modified Clickmers, clinically validate biomarkers, and introduce groundbreaking products that significantly enhance disease prediction and prevention.
